Microbiology Laboratory and Testing Methods

Topic	Laboratory Title	Methods
Basic Methods	Basic Microbiology Laboratory Concepts and Staining Procedures	Normal Flora Culture, Preparing a Smear, Gram Staining, Acid-Fast and Methylene Blue Staining, Antibiotic Sensitivity Testing
Differential Culture	Selective Media & Differential Culture of Bacteria	MSA Agar, S-S Agar, MAC and EMB agars
Staphylococcus	Identification of Gram-Positive Bacteria: The Normal Flora Staphylococci	Mannitol Salt Agar, Catalase Test
Streptococcus	Identification of Gram-Positive Bacteria: Differential Culture of the Streptococcal species	Hemolysis on Blood Agar, Bacitracin Sensitivity, CAMP test
Streptococcus	Throat swab for isolation of Streptococcal species	Blood agar culture and interpretation
Neisseriae and Haemophilus	Culture of Fastidious Organisms	Chocolate Agar, Satellitism with Staphylococcus, VX discs
Anaerobic Bacterial Culture	Signs and Symptoms of Anaerobic Infection and Sample Collection for Anaerobic Culture	Generating an Anaerobic Environment and Anaerobic Culture Systems
Gram Negative Enteric Bacteria	Culture and Identification of Gram Negative Enteric Organisms	Lactose Fermentation, MacConkey Agar, EMB, Enterotube